STS, SCAR and CAPS markers for identification of wheat resistance genes

Gene Marker Sequence of primers 5'-3' Reference Pm1 C320-1 C320-2 CCG GCA TAG ATC GAG AAT AG CCG GCA TAG AAC TTT AAG CG HU et al. 1997 Pm1 STS638-L STS638-R ACA GCG ATG AAG CAA TGA AA GTC CAG TTG GTT GAT GGA AT NEU et al. 2002 Pm2 Whs 350-1 Whs 350-2 AGC TGT TTG GGT ACA AGG TG TCC CCT GTG CTA CTA CTT CTC MOHLER, JAHOOR 1996 Pm3 N/A unpublished HARTL et al. 1993 Pm13 UTV 14F UTV 14R CGC CAG CCA ATT ATC TCC ATG A AGC CAT GCG CGG TGT CAT GTG AA CENCI et al. 1999 Lr1 pTAG621-5 pTAG621-3 GGG TCA CGT ACT ACT ATA CCT TGC CAG CCC AAA AG FEUILLET et al. 1995 Lr9 J 13/1 J 13/2 TCC TTT TAT TCC GCA CGC CGG CCA CAC TAC CCC AAA GAG ACG SCHACHERMAYR et al. 1997 Lr10 F1.2245 Lr10-6/r2 unpublished SCHACHERMAYR et al. 1997 Lr10 Thlr10G Thlr10H Thlr10J GCT CTT CTA ACG GGG ATC GGT AGA ACC GTG CCT TAC CAT GCT CTT GAA AGC TCC SCHACHERMAYR et al. 1994 Lrk10 Lrk10D1 Lrk10D2 Lrk10D3 Lrk10D4 GAA GCC CTT CGT CTC ATC TG TTG ATT CAT TGC CAG ATG AGA TCA CG GCT CGT CAT CTC CAC AGG ACC TCA TGC GGA TGT AG SCHACHERMAYR et al. 1997 Lrk10.6 N/A AAG ATC AAG AGG TAC CAT TGC TGG AAC CCG AGA AAC CGT CC SCHACHERMAYR et al. 1997 Lr19 GbF GbR CAT CCT TGG GGA CCT C CCA GCT CGC ATA CAT CCA PRINS et al. 2001 Lr19 SCS73719-1 SCS73719-2 TCG TCC AGA TCA GAA TGT G CTC GTCGATTAGCAGTGAG CHERUKURI et al. 2003 Lr20 STS638-L STS638-R ACA GCG ATG AAG CAA TGA AA GTC CAG TTG GTT GAT GGA AT NEU et al. 2002 Lr21 D14-L D14-R CGC TTT TAC C GA GAT TGG TC CCA AAG AGC ATC CAT GGT GT HUANG, GILL 2001 Lr21 D14L-N D14R-N CGA GAT TGG TCC TAT GAG GTG GT GGA CAT TAG GCG ATG CTT TGA AT HUANG et al. 2003 Lr24 J9/1 J9/2 TCT AGT CTG TAC ATG GGG GC TGG CAC ATG AAC TCC ATA CG SCHACHERMAYR et al. 1995 Lr24 SC-H51 SC-H52 AGT CGT CCC CGA AGA CCC GCT GGA TCG TCC CCT GAT GCC ATG TAA TGT DEDRYVER et al. 1996 Lr25 Lr25F20 Lr25R19 CCA CCC AGA GTA TAC CAG AG CCA CCC AGA GCT CAT AGA A PROCUNIER 2004 Lr26 (1RS/1BL trans.) J07IF1 J07IR1 TAA GCC GTA AAG CAT GGT GCA C CTT CAA CGA AAT GTT TTC CTC TTC IQBAL et al. 1995 Lr26 (1B/1R trans.) Xa1NBS-R XLRR-INV2 CTC TGT ATA CGA GTT GTC GAG GAA GGA CAG GTT GCC SHI et al. 2001 Lr26 (1BL/1RS trans.) O11B3 O11B5 SECA2 SECA3 GTT GCT GCT GAG GTT GGT TC GGT ACC AAC AAC AAC AAC CC GTT TGC TGG GGA ATT ATT TG TCC TCA TCT TTG TCC TCG CC FROIDMONT 1996 Lr28 Lr 28-01 Lr 28-02 CCC GGC ATA AGT CTA TGG TT CAA TGA ATG AGA TAC GTG AA NAIK et al. 1998 Lr29 OPY10/1 OPY10/2 unpublished TAR at al. 2002 Lr29 UBC219/1 UBC219/2 unpublished PROCUNIER et al. 1995, TAR at al. 2002 Lr29 Lr29F18 Lr29R18 Lr29F24 Lr29R24 GTG ACC TCA GGC AAT GCA GTG ACC TCA GAA CCG ATG GTG ACC TCA GGC AAT GCA CAC AGT GTG ACC TCA GAA CCG ATG TCC ATC PROCUNIER 2004 Lr35 BCD260F1 35R2 GAA GTT AAA GAG GTC TTG AC TTT TGA GAA TCA GTC ATC AC SEYFARTH et al. 1999 Lr35 Sr39F2 Sr39R3 AGA GAG AGT AGA AGA GCT GC AGA GAG AGA GCA TCC ACC GOLD et al. 1999 Lr37 Y15 F Y15 R unpublished SEAH et al. 2001 Lr37-Yr17-Sr38 cslVrgal3'F cslVrgal3'R unpublished SEAH et al. 2001 Lr37-Yr17-Sr38 VENTRIUP LN2 AGG GGC TAC TGA CCA AGG CT TGC AGC TAC AGC AGT ATG TAC ACA AAA HELGUERA et al. 2003 Lr37-Yr17-Sr38 URIC LN2 GGT CGC CCT GGC TTG CAC CT TGC AGC TAC AGC AGT ATG TAC ACA AAA HELGUERA et al. 2003 Lr47 PS10R PS10L GCT GAT GAC CCT GAC CGG T TCT TCA TGC CCG GTC GGG T HELGUERA et al. 2000 Lr47 PS10R PS10L2 GCT GAT GAC CCT GAC CGG T GGG CAG GCG TTT ATT CCA G HELGUERA et al. 2000 Lr47 PCAPSR PS10L PS10L2 CGT GAG ACT CGC CGT TAC CTT G TCT TCA TGC CCG GTC GGG T GGG CAG GCG TTT ATT CCA G HELGUERA et al. 2000 Lr51 S30-13L AGA7-759R GCA TCA ACA AGA TAT TCG TTA TGA CC TGG CTG CTC AGA AAA CTG GAC C HELGUERA et al. 2004 Cre3 RpEGF Kin2 GTG CTT CTT ATG AAC CCT TC TGA TAC TGG ATG ATG TCT GG SEAH et al. 1998

Sources: CENCI et al. 1999 Cenci A, D’Ovidio R, Tanzarella O A, Ceoloni C,Porceddu E "Identification of molekular markers linked to Pm13, an Aegilops longissima gene conferring resistance to powdery mildew in wheat." Theor. Appl. Genet. 98: 448-454; 1999 CHERUKURI et al. 2003 Cherukuri DP, Gupta SK, Charpe A, Koul S, Prabhu KV, Singh RB, Haq QMR, Chauhan VS "Identification of a molecular marker linked to an Agropyron elongatum-derived gene Lr19 for leaf rust resistance in wheat." Plant Breeding 122: 204-208; 2003 DEDRYVER et al. 1996 Dedryver F, Jubier MF, Thouverin J, Goyeau H "Molecular markers linked to leaf rust resistance gene Lr24 in different wheat cultivars." Genome 39: 830-835; 1996 FEUILLET et al. 1995 Feuillet C, Messmer M, Schachermayr G, Keller B "Genetical and physical characterization of the Lr1 leaf rust resistance locus in wheat (Triticum aestivum L.)." Mol. Gen. Genet. 248: 553-562; 1995 FROIDMONT 1998 Froidmont D "A co-dominant marker for the 1BL/1RS wheat-rye translocation via multiplex PCR." Journal of Cereal Science 27: 229-232; 1998 GOLD et al. 2002 Gold J, Harder D, Townley-Smith F, Aung T, Procunier J "Development of molecular marker for rust resistance genes Sr39 and Lr35 in wheat breeding lines." (2002) http://www.ejbiotechnology.info/content/vol2/issue HARTL et al. 1993 Hartl L, Weiss H, Zeller FJ, Jahoor A, "Use of RFLP markers for the identification of alleles of the Pm3 locus conferring powdery mildew resistance in wheat (Triticum aestivum L.)" Theor. Appl. Genet. 93: 959-963; 1993 HELGUERA et al. 2000 Helguera M, Khan IA, Dubcovsky J "Development of PCR markers for the wheat leaf rust resistance gene Lr47." Theor. Appl. Genet. 100:1137-1143; 2000 HELGUERA 2003 Helguera M "Disease resistance. Leaf rust. Lr47." (2003) http://maswheat.ucdavis.edu/protocols/lr39/index.htm HELGUERA et al. 2003 Helguera M, Khan IA, Kolmer J, Lijavetzky D, Zhong-Qi L, Dubcovsky J "PCR assays for the Lr37-Yr17-Sr38 cluster of rust resistance genes and their use to develop isogenic hard red spring wheat lines." Crop Sci 43: 1839-1847; 2003 HELGUERA et al. 2004 Helguera M, Vanzetti L, Soria M, Khan IA, Kolmer J, Dubcovsky J "PCR markers for Triticum speltoides leaf rust resistance gene Lr51 and their use to develop isogenic hard red spring wheat lines." (2004) http://maswheat.ucdavis.edu HU et al. 1997 Hu XY, Ohm HW, Dweikat J "Identification of RAPD markers linked to the gene Pm1 for resistance to powdery mildew in wheat." Theor. Appl. Genet. 94:832-840; 1997 HUANG et al. 2003 Huang L, Brooks SA, Li W, Fellers JP, Trick HN, Gill BS "Map-based cloning of leaf rust resistance gene Lr21 from the large and polyploid genome of bread wheat." Genetics 164:655-664; 2003 IQBAL et al. 1995 Iqbal MJ, Rayburn AL "Identification of the 1 RS rye of bread wheat chromosomal segment in wheat by RAPD analysis." Theor. Appl. Genet. 91:1048-1053; 1995 MOHLER, JAHOOR 1996 Mohler V, Jahoor A "Allele-specific amplification of polymorphic sites for the detection of powdery mildew resistance loci in cereals." Theor. Appl. Genet. 93:1078-1082; 1996 NAIK et al. 1998 Naik S, Gil KS, Prakasa RVS, Gupta VS, Tamhankar SA, Pujar S, Gill BS, Ranlekar PK "Identification of a STS marker linked to the Aegilops speltoides–derived leaf rust resistance gene Lr28 in wheat." Theor. Appl. Genet. 97:535-540; 1998 NEU et al. 2002 Neu CH, Stein N, Keller B "Genetic mapping of the Lr20-Pm1 resistance locus reveals suppressed recombination on chromosome arm 7AL in hexaploid wheat." Genome 45:737:744; 2002 PRINS et al. 2001 Prins R, Groenewald JZ, Marais GF, Snape JW, Koebner RMD "AFLP and STS tagging of Lr19, a gene conferring resistance to leaf rust in wheat." Theor. Appl. Genet. 103 618-624; 2001 PROCUNIER et al. 1995 Procunier JD, Townley-Smith TF, Prashar S, Gray M, Kim WK, Czarnecki E, Dyck PL "PCR-based RAPD/DGGE markers linked to leaf rust resistance genes Lr29 and Lr25 in wheat (Triticum aestivum L.)." J. Genet. Breed. 49:87-89; 1995 SCHACHERMAYR et al. 1994 Schachermayr G, Siedler H, Gale MD, Winzeler H, Winzeler RM, Keller B "Identification and localization of molecular markers linked to the Lr9 leaf rust resistance gene of wheat." Theor. Appl. Genet. 88:110-115; 1994 SCHACHERMAYR et al. 1995 Schachermayr G, Messmer M, Feuillet C, Winzeler H, Keller B "Identification of molecular markers linked to the Agropyron elongatum–derived leaf rust resistance gene Lr24 in wheat." Theor. Appl. Genet. 90:982-990; 1995 SCHACHERMAYR et al. 1997 Schachermayr G, Feuillet C, Keller B "Molecular markers for the detection of the wheat leaf rust resistance gene Lr10 in diverse genetic backgrounds." Mol. Breed. 3:65-74; 1997 SEAH et al. 1998 Seah S, Sivasithamparam K, Karakousis A, Lagudah ES "Cloning and characterization of a family of disease resistance gene analogs from wheat and barley." Theor.Appl.Genet. 97:937-945; 1998 SEAH et al.2003 Seah S, Bariana H, Jahier J, Sivasithamparam K, Lagudah ES "The introgressed segment carrying rust resistance genes Yr17, Lr37 and Sr38 in wheat can be assayed by a cloned disease resistance gene-like sequence." Theor. Appl. Genet. 102:600-605; 2001 SEYFARTH et al. 1999 Seyfarth R, Feuillet C, Schachermayr G, Winzeler RM, Keller B "Development of a molecular marker for the adult plant leaf rust resistance gene Lr35 in wheat." Theor. Appl. Genet. 99:554-560; 1999 SHI et al.2001 Shi ZX, Chen XM, Line RF, Leung H, Wellings CR "Development of resistance gene analog polymorphism markers for the Yr 9 gene resistance to wheat stripe rust." Genome 44: 509-516; 2001 TAR et al.2002 Tar M, Purnhauser L,Csösz L, Mesterházy A, Gyulai G "Identification of molecular markers for an efficient leaf rust resistnce gene (Lr29) in wheat." Acta Biologica Szegediensis 46:133-134; 2002